mouse imaging include chicken anti gfp Search Results


165ho edta mn1020 thermofisher plp mouse  (Thermo Fisher)
99
Thermo Fisher 165ho edta mn1020 thermofisher plp mouse
165ho Edta Mn1020 Thermofisher Plp Mouse, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+imaging+include+chicken+anti+gfp/Ethylenediaminetetraacetic+acid/pmc10462763__41467_2023_40937_MOESM1_ESM-140-112-115
Average 99 stars, based on 1 article reviews
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image studio software  (LI-COR)
98
LI-COR image studio software
Image Studio Software, supplied by LI-COR, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+imaging+include+chicken+anti+gfp/Fc+IMAGE+STUDIO+SOFTWARE/pmc08408659-63-6-9
Average 98 stars, based on 1 article reviews
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plko pgk rtta p2a cre sbe nls mscarlet  (Addgene inc)
95
Addgene inc plko pgk rtta p2a cre sbe nls mscarlet
Key resources table
Plko Pgk Rtta P2a Cre Sbe Nls Mscarlet, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+imaging+include+chicken+anti+gfp/pmScarlet-i_C1+(Plasmid+%2385044)/pmc08661530-420-176-170
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mmz stack images  (Nikon)
99
Nikon mmz stack images
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Mmz Stack Images, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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imaginal discs  (Bio-Rad)
92
Bio-Rad imaginal discs
Key resources table
Imaginal Discs, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dapi  (Thermo Fisher)
99
Thermo Fisher dapi
a Live-cell fluorescence imaging of stage II-V gametocytes transfected with the nuclear localisation signal (NLS)-mCherry reporter. NLS-mCherry (NLS-mCh, red) labels the elongated nucleus, with pointed extensions (yellow arrowheads). Subpellicular (green arrowheads) and nuclear (cyan arrowheads) populations of microtubules are labelled with Tubulin Tracker (TT, cyan). The nucleus contracts to a more spherical morphology and the nuclear and sub-pellicular microtubule populations are disassembled in stage V gametocytes. The chromatin (Hoechst, grayscale) is present as punctate features in the nucleus (white arrowheads). b Live-cell fluorescence imaging of stage II/III gametocytes in the Pf EB1-GFP/NLS-mCherry <t>co-transfectant</t> <t>parasite</t> line. NLS-mCherry (NLS-mCh, red) delineates the nucleus; and Pf EB1-GFP (EB1-GFP, green) is in the same compartment. Chromatin (Hoechst, grayscale) is distributed along the nuclear microtubules. (See Tubulin Tracker (TT, cyan) overlap with Pf EB1-GFP, cyan arrows). Additional images are presented in Supplementary Fig. . c Quantitative analysis of the contour lengths for the Pf EB1-GFP (EB1) and nuclear microtubules (MT) labelled with Tubulin Tracker (mean ± SD is shown; n = 20 cells per stage). Differences in the length of nuclear microtubule features were evaluated by two-way ANOVA Tukey’s test, **** p < 0.0001. The blue dashed line indicates the limit of resolution (250 nm) of the microscope. d Images showing that the nuclear microtubules (as marked by anti-GFP ( Pf EB1-GFP), green) are depolymerised and the subpellicular microtubules (white arrowheads) are modified with polyglutamate (polyE, red) in stage IV gametocytes. Anti-β-tubulin (anti-β-tub, magenta) labels both nuclear and subpellicular microtubules. The chromatin was labelled with <t>DAPI</t> (grayscale). e Quantitative analysis of anti-polyE fluorescence intensity at different stages of development (mean ± SD is shown; n = 15 cells). Differences in polyE fluorescence signal were determined using a paired one-way ANOVA Tukey’s, I vs. II and II vs. IIIa: NS > 0.9999; IIIa vs. IIIb, IIIb vs. IV and IV vs. V: **** p < 0.0001. Differential interference contrast (DIC) images are shown. Scale bars: 2 μm. Additional images are presented in Supplementary Fig. . Source data for Fig. c, e is provided in the Source Data file.
Dapi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+imaging+include+chicken+anti+gfp/PBS/pmc09419145-336-5-10
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mouse anti cc1  (Novus Biologicals)
92
Novus Biologicals mouse anti cc1
a Live-cell fluorescence imaging of stage II-V gametocytes transfected with the nuclear localisation signal (NLS)-mCherry reporter. NLS-mCherry (NLS-mCh, red) labels the elongated nucleus, with pointed extensions (yellow arrowheads). Subpellicular (green arrowheads) and nuclear (cyan arrowheads) populations of microtubules are labelled with Tubulin Tracker (TT, cyan). The nucleus contracts to a more spherical morphology and the nuclear and sub-pellicular microtubule populations are disassembled in stage V gametocytes. The chromatin (Hoechst, grayscale) is present as punctate features in the nucleus (white arrowheads). b Live-cell fluorescence imaging of stage II/III gametocytes in the Pf EB1-GFP/NLS-mCherry <t>co-transfectant</t> <t>parasite</t> line. NLS-mCherry (NLS-mCh, red) delineates the nucleus; and Pf EB1-GFP (EB1-GFP, green) is in the same compartment. Chromatin (Hoechst, grayscale) is distributed along the nuclear microtubules. (See Tubulin Tracker (TT, cyan) overlap with Pf EB1-GFP, cyan arrows). Additional images are presented in Supplementary Fig. . c Quantitative analysis of the contour lengths for the Pf EB1-GFP (EB1) and nuclear microtubules (MT) labelled with Tubulin Tracker (mean ± SD is shown; n = 20 cells per stage). Differences in the length of nuclear microtubule features were evaluated by two-way ANOVA Tukey’s test, **** p < 0.0001. The blue dashed line indicates the limit of resolution (250 nm) of the microscope. d Images showing that the nuclear microtubules (as marked by anti-GFP ( Pf EB1-GFP), green) are depolymerised and the subpellicular microtubules (white arrowheads) are modified with polyglutamate (polyE, red) in stage IV gametocytes. Anti-β-tubulin (anti-β-tub, magenta) labels both nuclear and subpellicular microtubules. The chromatin was labelled with <t>DAPI</t> (grayscale). e Quantitative analysis of anti-polyE fluorescence intensity at different stages of development (mean ± SD is shown; n = 15 cells). Differences in polyE fluorescence signal were determined using a paired one-way ANOVA Tukey’s, I vs. II and II vs. IIIa: NS > 0.9999; IIIa vs. IIIb, IIIb vs. IV and IV vs. V: **** p < 0.0001. Differential interference contrast (DIC) images are shown. Scale bars: 2 μm. Additional images are presented in Supplementary Fig. . Source data for Fig. c, e is provided in the Source Data file.
Mouse Anti Cc1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+imaging+include+chicken+anti+gfp/COPB2+Antibody+(M3A5)/pmc09170029-411-26-30
Average 92 stars, based on 1 article reviews
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horseradish peroxidase conjugated antibodies  (Thermo Fisher)
99
Thermo Fisher horseradish peroxidase conjugated antibodies
a Live-cell fluorescence imaging of stage II-V gametocytes transfected with the nuclear localisation signal (NLS)-mCherry reporter. NLS-mCherry (NLS-mCh, red) labels the elongated nucleus, with pointed extensions (yellow arrowheads). Subpellicular (green arrowheads) and nuclear (cyan arrowheads) populations of microtubules are labelled with Tubulin Tracker (TT, cyan). The nucleus contracts to a more spherical morphology and the nuclear and sub-pellicular microtubule populations are disassembled in stage V gametocytes. The chromatin (Hoechst, grayscale) is present as punctate features in the nucleus (white arrowheads). b Live-cell fluorescence imaging of stage II/III gametocytes in the Pf EB1-GFP/NLS-mCherry <t>co-transfectant</t> <t>parasite</t> line. NLS-mCherry (NLS-mCh, red) delineates the nucleus; and Pf EB1-GFP (EB1-GFP, green) is in the same compartment. Chromatin (Hoechst, grayscale) is distributed along the nuclear microtubules. (See Tubulin Tracker (TT, cyan) overlap with Pf EB1-GFP, cyan arrows). Additional images are presented in Supplementary Fig. . c Quantitative analysis of the contour lengths for the Pf EB1-GFP (EB1) and nuclear microtubules (MT) labelled with Tubulin Tracker (mean ± SD is shown; n = 20 cells per stage). Differences in the length of nuclear microtubule features were evaluated by two-way ANOVA Tukey’s test, **** p < 0.0001. The blue dashed line indicates the limit of resolution (250 nm) of the microscope. d Images showing that the nuclear microtubules (as marked by anti-GFP ( Pf EB1-GFP), green) are depolymerised and the subpellicular microtubules (white arrowheads) are modified with polyglutamate (polyE, red) in stage IV gametocytes. Anti-β-tubulin (anti-β-tub, magenta) labels both nuclear and subpellicular microtubules. The chromatin was labelled with <t>DAPI</t> (grayscale). e Quantitative analysis of anti-polyE fluorescence intensity at different stages of development (mean ± SD is shown; n = 15 cells). Differences in polyE fluorescence signal were determined using a paired one-way ANOVA Tukey’s, I vs. II and II vs. IIIa: NS > 0.9999; IIIa vs. IIIb, IIIb vs. IV and IV vs. V: **** p < 0.0001. Differential interference contrast (DIC) images are shown. Scale bars: 2 μm. Additional images are presented in Supplementary Fig. . Source data for Fig. c, e is provided in the Source Data file.
Horseradish Peroxidase Conjugated Antibodies, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+imaging+include+chicken+anti+gfp/PEROXIDASE+HORSERADISH/pm38713765-347-3-7
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horseradish peroxidase conjugated antibodies - by Bioz Stars, 2026-09
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cy3 anti mouse  (Jackson Immuno)
93
Jackson Immuno cy3 anti mouse
a Live-cell fluorescence imaging of stage II-V gametocytes transfected with the nuclear localisation signal (NLS)-mCherry reporter. NLS-mCherry (NLS-mCh, red) labels the elongated nucleus, with pointed extensions (yellow arrowheads). Subpellicular (green arrowheads) and nuclear (cyan arrowheads) populations of microtubules are labelled with Tubulin Tracker (TT, cyan). The nucleus contracts to a more spherical morphology and the nuclear and sub-pellicular microtubule populations are disassembled in stage V gametocytes. The chromatin (Hoechst, grayscale) is present as punctate features in the nucleus (white arrowheads). b Live-cell fluorescence imaging of stage II/III gametocytes in the Pf EB1-GFP/NLS-mCherry <t>co-transfectant</t> <t>parasite</t> line. NLS-mCherry (NLS-mCh, red) delineates the nucleus; and Pf EB1-GFP (EB1-GFP, green) is in the same compartment. Chromatin (Hoechst, grayscale) is distributed along the nuclear microtubules. (See Tubulin Tracker (TT, cyan) overlap with Pf EB1-GFP, cyan arrows). Additional images are presented in Supplementary Fig. . c Quantitative analysis of the contour lengths for the Pf EB1-GFP (EB1) and nuclear microtubules (MT) labelled with Tubulin Tracker (mean ± SD is shown; n = 20 cells per stage). Differences in the length of nuclear microtubule features were evaluated by two-way ANOVA Tukey’s test, **** p < 0.0001. The blue dashed line indicates the limit of resolution (250 nm) of the microscope. d Images showing that the nuclear microtubules (as marked by anti-GFP ( Pf EB1-GFP), green) are depolymerised and the subpellicular microtubules (white arrowheads) are modified with polyglutamate (polyE, red) in stage IV gametocytes. Anti-β-tubulin (anti-β-tub, magenta) labels both nuclear and subpellicular microtubules. The chromatin was labelled with <t>DAPI</t> (grayscale). e Quantitative analysis of anti-polyE fluorescence intensity at different stages of development (mean ± SD is shown; n = 15 cells). Differences in polyE fluorescence signal were determined using a paired one-way ANOVA Tukey’s, I vs. II and II vs. IIIa: NS > 0.9999; IIIa vs. IIIb, IIIb vs. IV and IV vs. V: **** p < 0.0001. Differential interference contrast (DIC) images are shown. Scale bars: 2 μm. Additional images are presented in Supplementary Fig. . Source data for Fig. c, e is provided in the Source Data file.
Cy3 Anti Mouse, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+imaging+include+chicken+anti+gfp/Cy+3+IgG+Fraction+Monoclonal+Mouse+Anti-Digoxin/bio_rxiv__2023__05__05__539603-209-8-14
Average 93 stars, based on 1 article reviews
cy3 anti mouse - by Bioz Stars, 2026-09
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hrp conjugated rabbit anti mouse igg secondary ab  (Bio-Rad)
94
Bio-Rad hrp conjugated rabbit anti mouse igg secondary ab
a Live-cell fluorescence imaging of stage II-V gametocytes transfected with the nuclear localisation signal (NLS)-mCherry reporter. NLS-mCherry (NLS-mCh, red) labels the elongated nucleus, with pointed extensions (yellow arrowheads). Subpellicular (green arrowheads) and nuclear (cyan arrowheads) populations of microtubules are labelled with Tubulin Tracker (TT, cyan). The nucleus contracts to a more spherical morphology and the nuclear and sub-pellicular microtubule populations are disassembled in stage V gametocytes. The chromatin (Hoechst, grayscale) is present as punctate features in the nucleus (white arrowheads). b Live-cell fluorescence imaging of stage II/III gametocytes in the Pf EB1-GFP/NLS-mCherry <t>co-transfectant</t> <t>parasite</t> line. NLS-mCherry (NLS-mCh, red) delineates the nucleus; and Pf EB1-GFP (EB1-GFP, green) is in the same compartment. Chromatin (Hoechst, grayscale) is distributed along the nuclear microtubules. (See Tubulin Tracker (TT, cyan) overlap with Pf EB1-GFP, cyan arrows). Additional images are presented in Supplementary Fig. . c Quantitative analysis of the contour lengths for the Pf EB1-GFP (EB1) and nuclear microtubules (MT) labelled with Tubulin Tracker (mean ± SD is shown; n = 20 cells per stage). Differences in the length of nuclear microtubule features were evaluated by two-way ANOVA Tukey’s test, **** p < 0.0001. The blue dashed line indicates the limit of resolution (250 nm) of the microscope. d Images showing that the nuclear microtubules (as marked by anti-GFP ( Pf EB1-GFP), green) are depolymerised and the subpellicular microtubules (white arrowheads) are modified with polyglutamate (polyE, red) in stage IV gametocytes. Anti-β-tubulin (anti-β-tub, magenta) labels both nuclear and subpellicular microtubules. The chromatin was labelled with <t>DAPI</t> (grayscale). e Quantitative analysis of anti-polyE fluorescence intensity at different stages of development (mean ± SD is shown; n = 15 cells). Differences in polyE fluorescence signal were determined using a paired one-way ANOVA Tukey’s, I vs. II and II vs. IIIa: NS > 0.9999; IIIa vs. IIIb, IIIb vs. IV and IV vs. V: **** p < 0.0001. Differential interference contrast (DIC) images are shown. Scale bars: 2 μm. Additional images are presented in Supplementary Fig. . Source data for Fig. c, e is provided in the Source Data file.
Hrp Conjugated Rabbit Anti Mouse Igg Secondary Ab, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+imaging+include+chicken+anti+gfp/Rabbit+anti+Mouse+IgG/pm28223253-62-6-22
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live imaging false fluorescent neurotransmitter 200 tocris 5911  (Tocris)
93
Tocris live imaging false fluorescent neurotransmitter 200 tocris 5911
a Live-cell fluorescence imaging of stage II-V gametocytes transfected with the nuclear localisation signal (NLS)-mCherry reporter. NLS-mCherry (NLS-mCh, red) labels the elongated nucleus, with pointed extensions (yellow arrowheads). Subpellicular (green arrowheads) and nuclear (cyan arrowheads) populations of microtubules are labelled with Tubulin Tracker (TT, cyan). The nucleus contracts to a more spherical morphology and the nuclear and sub-pellicular microtubule populations are disassembled in stage V gametocytes. The chromatin (Hoechst, grayscale) is present as punctate features in the nucleus (white arrowheads). b Live-cell fluorescence imaging of stage II/III gametocytes in the Pf EB1-GFP/NLS-mCherry <t>co-transfectant</t> <t>parasite</t> line. NLS-mCherry (NLS-mCh, red) delineates the nucleus; and Pf EB1-GFP (EB1-GFP, green) is in the same compartment. Chromatin (Hoechst, grayscale) is distributed along the nuclear microtubules. (See Tubulin Tracker (TT, cyan) overlap with Pf EB1-GFP, cyan arrows). Additional images are presented in Supplementary Fig. . c Quantitative analysis of the contour lengths for the Pf EB1-GFP (EB1) and nuclear microtubules (MT) labelled with Tubulin Tracker (mean ± SD is shown; n = 20 cells per stage). Differences in the length of nuclear microtubule features were evaluated by two-way ANOVA Tukey’s test, **** p < 0.0001. The blue dashed line indicates the limit of resolution (250 nm) of the microscope. d Images showing that the nuclear microtubules (as marked by anti-GFP ( Pf EB1-GFP), green) are depolymerised and the subpellicular microtubules (white arrowheads) are modified with polyglutamate (polyE, red) in stage IV gametocytes. Anti-β-tubulin (anti-β-tub, magenta) labels both nuclear and subpellicular microtubules. The chromatin was labelled with <t>DAPI</t> (grayscale). e Quantitative analysis of anti-polyE fluorescence intensity at different stages of development (mean ± SD is shown; n = 15 cells). Differences in polyE fluorescence signal were determined using a paired one-way ANOVA Tukey’s, I vs. II and II vs. IIIa: NS > 0.9999; IIIa vs. IIIb, IIIb vs. IV and IV vs. V: **** p < 0.0001. Differential interference contrast (DIC) images are shown. Scale bars: 2 μm. Additional images are presented in Supplementary Fig. . Source data for Fig. c, e is provided in the Source Data file.
Live Imaging False Fluorescent Neurotransmitter 200 Tocris 5911, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+imaging+include+chicken+anti+gfp/FFN+200+dihydrochloride/pmc08876465__jciinsight___7___151892___s209-263-108-114
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live imaging false fluorescent neurotransmitter 200 tocris 5911 - by Bioz Stars, 2026-09
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antibody antigen complexes  (Bio-Rad)
96
Bio-Rad antibody antigen complexes
a Live-cell fluorescence imaging of stage II-V gametocytes transfected with the nuclear localisation signal (NLS)-mCherry reporter. NLS-mCherry (NLS-mCh, red) labels the elongated nucleus, with pointed extensions (yellow arrowheads). Subpellicular (green arrowheads) and nuclear (cyan arrowheads) populations of microtubules are labelled with Tubulin Tracker (TT, cyan). The nucleus contracts to a more spherical morphology and the nuclear and sub-pellicular microtubule populations are disassembled in stage V gametocytes. The chromatin (Hoechst, grayscale) is present as punctate features in the nucleus (white arrowheads). b Live-cell fluorescence imaging of stage II/III gametocytes in the Pf EB1-GFP/NLS-mCherry <t>co-transfectant</t> <t>parasite</t> line. NLS-mCherry (NLS-mCh, red) delineates the nucleus; and Pf EB1-GFP (EB1-GFP, green) is in the same compartment. Chromatin (Hoechst, grayscale) is distributed along the nuclear microtubules. (See Tubulin Tracker (TT, cyan) overlap with Pf EB1-GFP, cyan arrows). Additional images are presented in Supplementary Fig. . c Quantitative analysis of the contour lengths for the Pf EB1-GFP (EB1) and nuclear microtubules (MT) labelled with Tubulin Tracker (mean ± SD is shown; n = 20 cells per stage). Differences in the length of nuclear microtubule features were evaluated by two-way ANOVA Tukey’s test, **** p < 0.0001. The blue dashed line indicates the limit of resolution (250 nm) of the microscope. d Images showing that the nuclear microtubules (as marked by anti-GFP ( Pf EB1-GFP), green) are depolymerised and the subpellicular microtubules (white arrowheads) are modified with polyglutamate (polyE, red) in stage IV gametocytes. Anti-β-tubulin (anti-β-tub, magenta) labels both nuclear and subpellicular microtubules. The chromatin was labelled with <t>DAPI</t> (grayscale). e Quantitative analysis of anti-polyE fluorescence intensity at different stages of development (mean ± SD is shown; n = 15 cells). Differences in polyE fluorescence signal were determined using a paired one-way ANOVA Tukey’s, I vs. II and II vs. IIIa: NS > 0.9999; IIIa vs. IIIb, IIIb vs. IV and IV vs. V: **** p < 0.0001. Differential interference contrast (DIC) images are shown. Scale bars: 2 μm. Additional images are presented in Supplementary Fig. . Source data for Fig. c, e is provided in the Source Data file.
Antibody Antigen Complexes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Key resources table

Journal: iScience

Article Title: A mechanistic basis for the malignant progression of salivary gland tumors

doi: 10.1016/j.isci.2021.103508

Figure Lengend Snippet: Key resources table

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken polyclonal anti-GFP Abcam Ab13970; RRID: AB_300798 Rabbit monoclonal anti-E-cadherin (clone 24E10) Cell Signaling Technology 3195; RRID: AB_2291471 Goat polyclonal anti-HMGA2 R&D Systems AF3184 Goat polyclonal anti-SOX2 R&D Systems AF2018; RRID: AB_355110 Mouse monoclonal anti-Vimentin Developmental Studies Hybridoma Bank 40E-C; RRID: AB_528504 Rabbit polyclonal anti-Aquaporin 5 Millipore AB15858; RRID: AB_992731 Rat monoclonal anti-Keratin 19 Developmental Studies Hybridoma Bank TROMA-III; RRID: AB_2133570 Rabbit polyclonal anti-Active Caspase-3 R&D Systems AF835; RRID: AB_2243952 Rat monoclonal anti-integrin α6 (clone GoH3) BD Biosciences 555734; RRID: AB_2296273 Chemicals, peptides, and recombinant proteins 5-ethynyl-2’-deoxyuridine (EdU) ThermoFisher Scientific A10044 Tamoxifen Sigma T5648 Critical commercial assays Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 488 dye ThermoFisher Scientific C10337 Experimental models: Cell lines Lenti-X 293T cell line Takara/Clontech 632180 Experimental models: Organisms/strains Tg(tetO-HRAS)65Lc NCI Mouse Repository Stock No. 01XB4 Gt(ROSA)26Sor tm1(EYFP)Cos The Jackson Laboratory Stock No. 006148 Recombinant DNA pMD2.G Dr. Didier Trono Addgene #12259 psPAX2 Dr. Didier Trono Addgene #12260 pMB80 (R26-CreER) Dr. Tyler Jacks Addgene #12168 pmScarlet-i_C1 Dr. Dorus Gadella Addgene #85044 pLKO-PGK-rtTA-P2A-Cre This paper n/a pLKO-PGK-rtTA-P2A-Cre-SBE-NLS-mScarlet-I Taniguchi et al., 2020 n/a pLKO-PGK-rtTA-SBE-NLS-mScarlet-I-P2A-CreER This paper n/a Software and algorithms Fiji https://fiji.sc/ GraphPad Prism 9 GraphPad Software, LLC www.graphpad.com Other The Vevo2100 High-Resolution Micro-Imaging System FUJIFILM VisualSonics VS-20047 Nanoject III Auto-nanoliter injector Drummond Scientific Company 3-000-207 Open in a separate window Key resources table

Techniques: Recombinant, Imaging, Software

a Live-cell fluorescence imaging of stage II-V gametocytes transfected with the nuclear localisation signal (NLS)-mCherry reporter. NLS-mCherry (NLS-mCh, red) labels the elongated nucleus, with pointed extensions (yellow arrowheads). Subpellicular (green arrowheads) and nuclear (cyan arrowheads) populations of microtubules are labelled with Tubulin Tracker (TT, cyan). The nucleus contracts to a more spherical morphology and the nuclear and sub-pellicular microtubule populations are disassembled in stage V gametocytes. The chromatin (Hoechst, grayscale) is present as punctate features in the nucleus (white arrowheads). b Live-cell fluorescence imaging of stage II/III gametocytes in the Pf EB1-GFP/NLS-mCherry co-transfectant parasite line. NLS-mCherry (NLS-mCh, red) delineates the nucleus; and Pf EB1-GFP (EB1-GFP, green) is in the same compartment. Chromatin (Hoechst, grayscale) is distributed along the nuclear microtubules. (See Tubulin Tracker (TT, cyan) overlap with Pf EB1-GFP, cyan arrows). Additional images are presented in Supplementary Fig. . c Quantitative analysis of the contour lengths for the Pf EB1-GFP (EB1) and nuclear microtubules (MT) labelled with Tubulin Tracker (mean ± SD is shown; n = 20 cells per stage). Differences in the length of nuclear microtubule features were evaluated by two-way ANOVA Tukey’s test, **** p < 0.0001. The blue dashed line indicates the limit of resolution (250 nm) of the microscope. d Images showing that the nuclear microtubules (as marked by anti-GFP ( Pf EB1-GFP), green) are depolymerised and the subpellicular microtubules (white arrowheads) are modified with polyglutamate (polyE, red) in stage IV gametocytes. Anti-β-tubulin (anti-β-tub, magenta) labels both nuclear and subpellicular microtubules. The chromatin was labelled with DAPI (grayscale). e Quantitative analysis of anti-polyE fluorescence intensity at different stages of development (mean ± SD is shown; n = 15 cells). Differences in polyE fluorescence signal were determined using a paired one-way ANOVA Tukey’s, I vs. II and II vs. IIIa: NS > 0.9999; IIIa vs. IIIb, IIIb vs. IV and IV vs. V: **** p < 0.0001. Differential interference contrast (DIC) images are shown. Scale bars: 2 μm. Additional images are presented in Supplementary Fig. . Source data for Fig. c, e is provided in the Source Data file.

Journal: Nature Communications

Article Title: Repurposing the mitotic machinery to drive cellular elongation and chromatin reorganisation in Plasmodium falciparum gametocytes

doi: 10.1038/s41467-022-32579-4

Figure Lengend Snippet: a Live-cell fluorescence imaging of stage II-V gametocytes transfected with the nuclear localisation signal (NLS)-mCherry reporter. NLS-mCherry (NLS-mCh, red) labels the elongated nucleus, with pointed extensions (yellow arrowheads). Subpellicular (green arrowheads) and nuclear (cyan arrowheads) populations of microtubules are labelled with Tubulin Tracker (TT, cyan). The nucleus contracts to a more spherical morphology and the nuclear and sub-pellicular microtubule populations are disassembled in stage V gametocytes. The chromatin (Hoechst, grayscale) is present as punctate features in the nucleus (white arrowheads). b Live-cell fluorescence imaging of stage II/III gametocytes in the Pf EB1-GFP/NLS-mCherry co-transfectant parasite line. NLS-mCherry (NLS-mCh, red) delineates the nucleus; and Pf EB1-GFP (EB1-GFP, green) is in the same compartment. Chromatin (Hoechst, grayscale) is distributed along the nuclear microtubules. (See Tubulin Tracker (TT, cyan) overlap with Pf EB1-GFP, cyan arrows). Additional images are presented in Supplementary Fig. . c Quantitative analysis of the contour lengths for the Pf EB1-GFP (EB1) and nuclear microtubules (MT) labelled with Tubulin Tracker (mean ± SD is shown; n = 20 cells per stage). Differences in the length of nuclear microtubule features were evaluated by two-way ANOVA Tukey’s test, **** p < 0.0001. The blue dashed line indicates the limit of resolution (250 nm) of the microscope. d Images showing that the nuclear microtubules (as marked by anti-GFP ( Pf EB1-GFP), green) are depolymerised and the subpellicular microtubules (white arrowheads) are modified with polyglutamate (polyE, red) in stage IV gametocytes. Anti-β-tubulin (anti-β-tub, magenta) labels both nuclear and subpellicular microtubules. The chromatin was labelled with DAPI (grayscale). e Quantitative analysis of anti-polyE fluorescence intensity at different stages of development (mean ± SD is shown; n = 15 cells). Differences in polyE fluorescence signal were determined using a paired one-way ANOVA Tukey’s, I vs. II and II vs. IIIa: NS > 0.9999; IIIa vs. IIIb, IIIb vs. IV and IV vs. V: **** p < 0.0001. Differential interference contrast (DIC) images are shown. Scale bars: 2 μm. Additional images are presented in Supplementary Fig. . Source data for Fig. c, e is provided in the Source Data file.

Article Snippet: Parasite chromatin was labelled with DAPI (2 μg/mL in PBS, Thermo Scientific) for 15 minutes at room temperature, followed by washes with PBS buffer.

Techniques: Fluorescence, Imaging, Transfection, Cotransfection, Microscopy, Modification